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This volume and its companion, Volume 351, are specifically designed to meet the needs of graduate students and postdoctoral students as well as researchers, by providing all the up-to-date methods necessary to study genes in yeast. Procedures are included that enable newcomers to set up a yeast laboratory and to master basic manipulations. Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines. Specific topics addressed in this book include basic techniques, making mutants, genomics, and proteomics.

Genetic Techniques. --- Molecular Biology. --- Yeasts --- 582.282.23 --- 575 --- 577.2 --- Biochemical Genetics --- Biology, Molecular --- Genetics, Biochemical --- Genetics, Molecular --- Molecular Genetics --- Biochemical Genetic --- Genetic, Biochemical --- Genetic, Molecular --- Molecular Genetic --- Genetic Technic --- Genetic Technics --- Genetic Technique --- Technic, Genetic --- Technics, Genetic --- Technique, Genetic --- Techniques, Genetic --- Genetics --- Genetic Counseling --- Genetic Processes --- genetics. --- Saccharomycetineae. Yeasts. Brettanomyces --- General genetics. General cytogenetics. Immunogenetics. Evolution. Speciation. Phylogeny --- Molecular bases of life. Molecular biology --- methods --- Molecular biology. --- Saccharomyces cerevisiae. --- Yeast. --- Yeast fungi. --- Biochemistry --- Fungi --- Investigative Techniques --- Biology --- Biological Science Disciplines --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Chemistry --- Eukaryota --- Natural Science Disciplines --- Organisms --- Disciplines and Occupations --- Genetic Techniques --- Molecular Biology --- Genetic Phenomena --- 577.2 Molecular bases of life. Molecular biology --- 575 General genetics. General cytogenetics. Immunogenetics. Evolution. Speciation. Phylogeny --- 582.282.23 Saccharomycetineae. Yeasts. Brettanomyces --- Yeast --- Natural Sciences --- Physical Sciences --- Discipline, Natural Science --- Disciplines, Natural Science --- Natural Science --- Natural Science Discipline --- Physical Science --- Science, Natural --- Science, Physical --- Sciences, Natural --- Sciences, Physical --- Eucarya --- Eukarya --- Eukaryotes --- Eukaryotas --- Eukaryote --- Biologic Sciences --- Biological Science --- Science, Biological --- Sciences, Biological --- Biological Sciences --- Life Sciences --- Biologic Science --- Biological Science Discipline --- Discipline, Biological Science --- Disciplines, Biological Science --- Life Science --- Science Discipline, Biological --- Science Disciplines, Biological --- Science, Biologic --- Science, Life --- Sciences, Biologic --- Sciences, Life --- Investigative Technics --- Investigative Technic --- Investigative Technique --- Technic, Investigative --- Technics, Investigative --- Technique, Investigative --- Techniques, Investigative --- Fungi, Filamentous --- Molds --- Filamentous Fungi --- Filamentous Fungus --- Fungus --- Fungus, Filamentous --- Mold --- Fungal Proteins --- Genetic Structures --- Allergens --- Microbiology --- genetics --- Genomics. --- Cell Biology.

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As exciting as the new field of genomics is, it has not yet produced a basic conceptual change in biology. The fundamental problems remain: the origin of life, cell organization, the pathways of differentiation, aging, and the molecular and cellular capabilities of the brain. What has occurred is an explosion of molecular information obtained by genomic sequences, which will soon be followed by exhaustive catalogs of protein interactions and protein function. This wealth of information can be analyzed and manipulated only with the help of computers. The rapidly expanding role of computers in biology may usher in a profound conceptual change in how we study living systems in the laboratory. This book focuses on current computational approaches to understanding the complex networks of metabolic and gene regulatory capabilities of the cell. The contributors look well beyond the state of the art in computational biology to anticipate what biological research will be like in a post-genomic world.

Gene Expression Regulation --- Models, Genetic --- Metabolism --- Models, Biological --- Metabolic Phenomena --- Genetic Processes --- Phenomena and Processes --- Models, Theoretical --- Genetic Phenomena --- Investigative Techniques --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Genetics --- Biology --- Health & Biological Sciences --- Investigative Technics --- Investigative Technic --- Investigative Technique --- Technic, Investigative --- Technics, Investigative --- Technique, Investigative --- Techniques, Investigative --- Genetic Concepts --- Genetic Phenomenon --- Genetic Process --- Concept, Genetic --- Concepts, Genetic --- Genetic Concept --- Phenomena, Genetic --- Phenomenon, Genetic --- Process, Genetic --- Processes, Genetic --- Experimental Model --- Experimental Models --- Mathematical Model --- Model, Experimental --- Models (Theoretical) --- Models, Experimental --- Models, Theoretic --- Theoretical Study --- Mathematical Models --- Model (Theoretical) --- Model, Mathematical --- Model, Theoretical --- Models, Mathematical --- Studies, Theoretical --- Study, Theoretical --- Theoretical Model --- Theoretical Models --- Theoretical Studies --- Metabolic Phenomenon --- Metabolic Process --- Metabolism Concepts --- Metabolism Phenomena --- Process, Metabolic --- Processes, Metabolic --- Anabolism --- Catabolism --- Metabolic Concepts --- Metabolic Processes --- Concept, Metabolic --- Concept, Metabolism --- Concepts, Metabolic --- Concepts, Metabolism --- Metabolic Concept --- Metabolism Concept --- Phenomena, Metabolic --- Phenomena, Metabolism --- Phenomenon, Metabolic --- Biological Model --- Biological Models --- Model, Biological --- Models, Biologic --- Biologic Model --- Biologic Models --- Model, Biologic --- Genetic Models --- Genetic Model --- Model, Genetic --- Expression Regulation, Gene --- Regulation, Gene Action --- Regulation, Gene Expression --- Gene Action Regulation --- Regulation of Gene Expression --- Molecular Biology --- Computer Simulation --- Systems Theory --- RNAi Therapeutics --- Gene Regulatory Networks --- Molecular biology --- Mathematical models. --- BIOMEDICAL SCIENCES/Quantitative Biology --- Molecular biochemistry --- Molecular biophysics --- Biochemistry --- Biophysics --- Biomolecules --- Systems biology --- Biomathematics

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Saccharomyces cerevisiae --- Yeast fungi --- Levures (Botanique) --- cytology --- genetics --- enzymology --- Genetics --- Molecular aspects --- Cytology --- Génétique --- Levure --- Yeasts --- Génie génétique --- genetic engineering --- Cytologie --- Biochimie --- biochemistry --- Fungi --- Clinical Laboratory Techniques --- Biochemistry --- Biological Science Disciplines --- Chemistry --- Biology --- Eukaryota --- Investigative Techniques --- Organisms --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Natural Science Disciplines --- Disciplines and Occupations --- Molecular Biology --- Cytological Techniques --- Botany --- Earth & Environmental Sciences --- Fungi & Algae --- Genetic Techniques. --- Molecular Biology. --- 582.282.23 --- 575 --- 577.2 --- Biochemical Genetics --- Biology, Molecular --- Genetics, Biochemical --- Genetics, Molecular --- Molecular Genetics --- Biochemical Genetic --- Genetic, Biochemical --- Genetic, Molecular --- Molecular Genetic --- Genetic Technic --- Genetic Technics --- Genetic Technique --- Technic, Genetic --- Technics, Genetic --- Technique, Genetic --- Techniques, Genetic --- Genetic Counseling --- Genetic Processes --- genetics. --- Saccharomycetineae. Yeasts. Brettanomyces --- General genetics. General cytogenetics. Immunogenetics. Evolution. Speciation. Phylogeny --- Molecular bases of life. Molecular biology --- methods --- Genetic Phenomena --- 577.2 Molecular bases of life. Molecular biology --- 575 General genetics. General cytogenetics. Immunogenetics. Evolution. Speciation. Phylogeny --- 582.282.23 Saccharomycetineae. Yeasts. Brettanomyces --- Génétique --- Yeast --- Cytologic Technics --- Cytological Technic --- Cytological Technics --- Cytological Technique --- Technic, Cytological --- Technics, Cytological --- Technique, Cytological --- Techniques, Cytological --- Cytologic Technic --- Technic, Cytologic --- Technics, Cytologic --- Cell Biology --- Natural Sciences --- Physical Sciences --- Discipline, Natural Science --- Disciplines, Natural Science --- Natural Science --- Natural Science Discipline --- Physical Science --- Science, Natural --- Science, Physical --- Sciences, Natural --- Sciences, Physical --- Investigative Technics --- Investigative Technic --- Investigative Technique --- Technic, Investigative --- Technics, Investigative --- Technique, Investigative --- Techniques, Investigative --- Eucarya --- Eukarya --- Eukaryotes --- Eukaryotas --- Eukaryote --- Biologic Sciences --- Biological Science --- Science, Biological --- Sciences, Biological --- Biological Sciences --- Life Sciences --- Biologic Science --- Biological Science Discipline --- Discipline, Biological Science --- Disciplines, Biological Science --- Life Science --- Science Discipline, Biological --- Science Disciplines, Biological --- Science, Biologic --- Science, Life --- Sciences, Biologic --- Sciences, Life --- Diagnoses and Laboratory Examinations --- Diagnosis, Laboratory --- Laboratory Diagnosis --- Laboratory Examinations and Diagnoses --- Laboratory Techniques, Clinical --- Clinical Laboratory Technique --- Diagnoses, Laboratory --- Laboratory Diagnoses --- Laboratory Technique, Clinical --- Technique, Clinical Laboratory --- Techniques, Clinical Laboratory --- Fungi, Filamentous --- Molds --- Filamentous Fungi --- Filamentous Fungus --- Fungus --- Fungus, Filamentous --- Mold --- cytology. --- enzymology. --- Genetic Techniques --- Cell Biology. --- Yeasts - genetics --- Genomics. --- Proteomics --- Biologie cellulaire

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The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today?truly an essential publication for researchers in all fields of life sciences.Major Sections Include: * Basic Principles, Specialized Uses, and Genetic Applications* LC

Dissection. --- Lasers in biology. --- Microdissection. --- Microscopy. --- Surgical Procedures, Operative --- Specimen Handling --- Investigative Techniques --- Microscopy --- Optical Devices --- Radiation Equipment and Supplies --- Equipment and Supplies --- Diagnostic Imaging --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Laboratory Techniques and Procedures --- Diagnosis --- Diagnostic Techniques and Procedures --- Dissection --- Microscopy, Confocal --- Lasers --- Genetic Techniques --- Human Anatomy & Physiology --- Health & Biological Sciences --- Animal Biochemistry --- Continuous Wave Lasers --- Pulsed Lasers --- Q-Switched Lasers --- Masers --- Continuous Wave Laser --- Laser --- Laser, Continuous Wave --- Laser, Pulsed --- Laser, Q-Switched --- Lasers, Continuous Wave --- Lasers, Pulsed --- Lasers, Q-Switched --- Maser --- Pulsed Laser --- Q Switched Lasers --- Q-Switched Laser --- Confocal Laser Scanning Microscopy --- Confocal Microscopy --- Confocal Microscopy, Scanning Laser --- Laser Microscopy --- Laser Scanning Confocal Microscopy --- Laser Scanning Microscopy --- Microscopy, Confocal, Laser Scanning --- Confocal Microscopies --- Laser Microscopies --- Laser Scanning Microscopies --- Microscopies, Confocal --- Microscopies, Laser --- Microscopies, Laser Scanning --- Microscopy, Laser --- Microscopy, Laser Scanning --- Scanning Microscopies, Laser --- Scanning Microscopy, Laser --- Genetic Technic --- Genetic Technics --- Genetic Technique --- Technic, Genetic --- Technics, Genetic --- Technique, Genetic --- Techniques, Genetic --- Genetics --- Dissections --- Diagnostic Technics and Procedures --- Technics and Procedures, Diagnostic --- Techniques and Procedures, Diagnostic --- Imaging, Diagnostic --- Imaging, Medical --- Medical Imaging --- Device, Medical --- Devices, Medical --- Equipment --- Inventories --- Medical Device --- Supplies --- Apparatus and Instruments --- Devices --- Medical Devices --- Device --- Instruments and Apparatus --- Inventory --- Supplies and Equipment --- Optical Components --- Optical System --- Component, Optical --- Components, Optical --- Device, Optical --- Devices, Optical --- Optical Component --- Optical Device --- Optical Systems --- System, Optical --- Systems, Optical --- Microscopies --- Investigative Technics --- Investigative Technic --- Investigative Technique --- Technic, Investigative --- Technics, Investigative --- Technique, Investigative --- Techniques, Investigative --- Specimen Collection --- Collection, Specimen --- Collections, Specimen --- Handling, Specimen --- Handlings, Specimen --- Specimen Collections --- Specimen Handlings --- Ghost Surgery --- Operative Procedures --- Operative Surgical Procedure --- Operative Surgical Procedures --- Procedure, Operative Surgical --- Procedures, Operative Surgical --- Surgery, Ghost --- Surgical Procedure, Operative --- Operative Procedure --- Procedure, Operative --- Procedures, Operative --- Antemortem Diagnosis --- Diagnoses and Examinations --- Examinations and Diagnoses --- Postmortem Diagnosis --- Antemortem Diagnoses --- Diagnoses --- Diagnoses, Antemortem --- Diagnoses, Postmortem --- Diagnosis, Antemortem --- Diagnosis, Postmortem --- Postmortem Diagnoses --- Disease --- methods --- diagnosis --- Optical Tweezers --- Laser Therapy --- Genetic Counseling --- Genetic Phenomena --- Anatomy --- Sensitivity and Specificity --- Radiologic and Imaging Nursing --- Image Processing, Computer-Assisted --- Optics and Photonics --- Compound Microscopy --- Hand-Held Microscopy --- Light Microscopy --- Optical Microscopy --- Simple Microscopy --- Hand Held Microscopy --- Microscopy, Compound --- Microscopy, Hand-Held --- Microscopy, Light --- Microscopy, Optical --- Microscopy, Simple --- General Surgery --- Diagnostic Testing --- Testing, Diagnostic --- Diagnose --- Surgical Procedures --- Procedure, Surgical --- Procedures, Surgical --- Surgical Procedure --- Genetic Techniques. --- Lasers. --- methods.

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Allergens. --- Contact dermatitis. --- Dermatitis, Contact. --- Dermatitis, Occupational. --- Irritants. --- Occupational dermatitis. --- Patch Tests. --- Occupational dermatitis --- Contact dermatitis --- Skin Tests --- Noxae --- Antigens --- Skin Diseases, Eczematous --- Riot Control Agents, Chemical --- Dermatitis --- Specialty Uses of Chemicals --- Occupational Diseases --- Immunologic Tests --- Biological Factors --- Chemical Actions and Uses --- Skin Diseases --- Toxic Actions --- Diagnostic Techniques and Procedures --- Diseases --- Diagnosis --- Skin and Connective Tissue Diseases --- Chemicals and Drugs --- Immunologic Techniques --- Laboratory Techniques and Procedures --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Investigative Techniques --- Irritants --- Patch Tests --- Dermatitis, Contact --- Dermatitis, Occupational --- Allergens --- Medicine --- Health & Biological Sciences --- Dermatology --- Allergen --- Pollen --- Dermatosis, Industrial --- Industrial Dermatosis --- Occupational Dermatitis --- Dermatitides, Occupational --- Dermatoses, Industrial --- Industrial Dermatoses --- Occupational Dermatitides --- Contact Dermatitis --- Dermatitis Venenata --- Eczema, Contact --- Hypersensitivity, Contact --- Sensitivity, Contact --- Contact Dermatitides --- Contact Eczema --- Contact Hypersensitivities --- Contact Hypersensitivity --- Contact Sensitivities --- Contact Sensitivity --- Dermatitides, Contact --- Hypersensitivities, Contact --- Sensitivities, Contact --- Patch Test --- Test, Patch --- Tests, Patch --- Counterirritants --- Pustulants --- Rubefacients --- Vesicants --- Investigative Technics --- Investigative Technic --- Investigative Technique --- Technic, Investigative --- Technics, Investigative --- Technique, Investigative --- Techniques, Investigative --- Antibody Dissociation --- Immunologic Technic --- Immunologic Technics --- Immunologic Technique --- Immunological Technics --- Immunological Techniques --- Technic, Immunologic --- Technics, Immunologic --- Technique, Immunologic --- Techniques, Immunologic --- Antibody Dissociations --- Dissociation, Antibody --- Dissociations, Antibody --- Immunological Technic --- Immunological Technique --- Technic, Immunological --- Technics, Immunological --- Technique, Immunological --- Techniques, Immunological --- Allergy and Immunology --- Immunity --- Immunochemistry --- Antemortem Diagnosis --- Diagnoses and Examinations --- Examinations and Diagnoses --- Postmortem Diagnosis --- Antemortem Diagnoses --- Diagnoses --- Diagnoses, Antemortem --- Diagnoses, Postmortem --- Diagnosis, Antemortem --- Diagnosis, Postmortem --- Postmortem Diagnoses --- Disease --- Diagnostic Technics and Procedures --- Technics and Procedures, Diagnostic --- Techniques and Procedures, Diagnostic --- Sensitivity and Specificity --- Environmental Pollutants, Noxae, and Pesticides --- Actions, Toxic --- Dermatosis --- Dermatoses --- Skin and Subcutaneous Tissue Disorders --- Skin Disease --- Biologic Agents --- Biologic Factors --- Biological Factor --- Factor, Biologic --- Factor, Biological --- Factors, Biological --- Biological Agents --- Agents, Biologic --- Agents, Biological --- Biologic Factor --- Factors, Biologic --- Biological Products --- Diagnosis, Immunologic --- Immunologic Diagnosis --- Immunologic Test --- Immunological Tests --- Tests, Immunologic --- Diagnosis, Immunological --- Immunodiagnosis --- Diagnoses, Immunologic --- Diagnoses, Immunological --- Immunodiagnoses --- Immunologic Diagnoses --- Immunological Diagnoses --- Immunological Diagnosis --- Immunological Test --- Test, Immunologic --- Test, Immunological --- Tests, Immunological --- Diseases, Occupational --- Occupational Illnesses --- Disease, Occupational --- Illnesse, Occupational --- Illnesses, Occupational --- Occupational Disease --- Occupational Illnesse --- Accidents, Occupational --- Industry --- Occupational Medicine --- Karoshi Death --- Specialty Chemicals and Products --- Dermatitides --- Chemical Riot Control Agents --- Eczematous Disorders --- Eczematous Skin Diseases --- Disease, Eczematous Skin --- Diseases, Eczematous Skin --- Disorder, Eczematous --- Disorders, Eczematous --- Eczematous Disorder --- Eczematous Skin Disease --- Skin Disease, Eczematous --- Antigen --- Toxins, Biological --- Skin Test --- Test, Skin --- Tests, Skin --- Contact hypersensitivity --- Contact sensitivity --- Dermatitis venenata --- Delayed hypersensitivity --- Skin --- Industrial dermatitis --- Occupational diseases --- methods --- diagnosis --- Inflammation --- Patch Testing --- Patch Testings --- Testing, Patch --- Testings, Patch --- Counterirritant --- Irritant --- Pustulant --- Rubefacient --- Vesicant --- Diagnose --- Diagnostic Testing --- Testing, Diagnostic --- Toxic Action --- Action, Toxic --- Biologic Agent --- Biological Agent --- Agent, Biologic --- Agent, Biological --- irritants.